I am not familiar with that BOB but have you checked the pins assigned to each axis in Mach. each axis has 2 signal pins one for direction and the other for steps (or pulses) to operate the axis. There is also a backup file that Mach creates each time a change is made for the XML file info.
See attached and check the XML file for plasma in the main Mach3 folder to see the last change date, this will tell you if you changed something and that is why it is not working now. In the XML backup folder you will find the last backup file which has the setting before the last change. If you did make a change and need to correct the change you can remove the Plasma XML file from the Mach3 filer and then copy and rename the backup XML file to the Mach 3 main folder (plasma.XML) now if you are using another software package it may have a different name.
motor 3.JPG
XML file in the main Mach3 folder
motor XML.JPG
backup XML file in the backup XML folder
motor pins.JPG
Axis signal pins in Motor driver output